| # | Team | P | W | D | L | GF | GA | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Cheltenham | 46 | 24 | 10 | 12 | 61 | 39 | +22 | 82 | WLDWW |
| 2 | Cambridge United | 46 | 24 | 8 | 14 | 73 | 49 | +24 | 80 | WLLWW |
| 3 | Bolton | 46 | 23 | 10 | 13 | 59 | 50 | +9 | 79 | WLWWL |
| 4 | Morecambe | 46 | 23 | 9 | 14 | 69 | 58 | +11 | 78 | WWLWW |
| 5 | Newport County | 46 | 20 | 13 | 13 | 57 | 42 | +15 | 73 | DWWDW |
| 6 | Forest Green | 46 | 20 | 13 | 13 | 59 | 51 | +8 | 73 | WWLDD |
| 7 | Tranmere | 46 | 20 | 13 | 13 | 55 | 50 | +5 | 73 | DLWWD |
| 8 | Salford City | 46 | 19 | 14 | 13 | 54 | 34 | +20 | 71 | WLWWW |
| 9 | Exeter City | 46 | 18 | 16 | 12 | 71 | 50 | +21 | 70 | DWWDD |
| 10 | Carlisle | 46 | 18 | 12 | 16 | 60 | 51 | +9 | 66 | DWDDL |
| 11 | Leyton Orient | 46 | 17 | 10 | 19 | 53 | 55 | -2 | 61 | LLLLW |
| 12 | Crawley Town | 46 | 16 | 13 | 17 | 56 | 62 | -6 | 61 | LDDLW |
| 13 | Port Vale | 46 | 17 | 9 | 20 | 57 | 57 | 0 | 60 | LLWWD |
| 14 | Stevenage | 46 | 14 | 18 | 14 | 41 | 41 | 0 | 60 | WDWLL |
| 15 | Bradford | 46 | 16 | 11 | 19 | 48 | 53 | -5 | 59 | LDLLL |
| 16 | Mansfield Town | 46 | 13 | 19 | 14 | 57 | 55 | +2 | 58 | WWLWW |
| 17 | Harrogate Town | 46 | 16 | 9 | 21 | 52 | 61 | -9 | 57 | LWDLW |
| 18 | Oldham | 46 | 15 | 9 | 22 | 72 | 81 | -9 | 54 | LLLWL |
| 19 | Walsall | 46 | 11 | 20 | 15 | 45 | 53 | -8 | 53 | DLWLL |
| 20 | Colchester | 46 | 11 | 18 | 17 | 44 | 61 | -17 | 51 | DWLWW |
| 21 | Barrow | 46 | 13 | 11 | 22 | 53 | 59 | -6 | 50 | DLWLL |
| 22 | Scunthorpe | 46 | 13 | 9 | 24 | 41 | 64 | -23 | 48 | LDLLL |
| 23 | Southend | 46 | 10 | 15 | 21 | 29 | 58 | -29 | 45 | DWWLD |
| 24 | Grimsby | 46 | 10 | 13 | 23 | 37 | 69 | -32 | 43 | LWLWL |
Player stats
League Two match stats only.
Bolton finished 3rd in the League Two in 2020/21 with 79 points (23W 10D 13L). They scored 59 and conceded 50, a goal difference of +9. Eoin Doyle top-scored with 19 goals and Gethin Jones and Arthur Gnahoua led the assists with 5 each. The final table, every result and the season's team statistics are below.